產(chǎn)品詳情介紹
DMT Enzyme為改良型的DpnI限制性內(nèi)切酶，與傳統(tǒng)的DpnI限制性內(nèi)切酶相比，具有更高的活性。該酶可以有效地識(shí)別并切割腺嘌呤甲基化的GmATC序列，而不能切割非甲基化的GATC序列。DMT Enzyme與多種PCR酶的反應(yīng)緩沖液兼容(如EasyPfu、TransStart FastPfu等)，PCR結(jié)束后，在反應(yīng)體系中直接加入該酶即可工作，反應(yīng)結(jié)束后，無需加熱失活即可進(jìn)行下游轉(zhuǎn)化實(shí)驗(yàn)。
1.Liu M , Feng Z , Ke H , et al. Tango1 spatially organizes ER exit sites to control ER export[J]. The Journal of Cell Biology, 2017, 216(4):1035-1049.
2.Bingqing L , Yingying Y , Yajie Z , et al. A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates Pathogenicity[J]. Frontiers in Cellular and Infection Microbiology, 2017, 7:26.
3.Wang T N , Zhao M . A simple strategy for extracellular production of CotA laccase in Escherichia coli, and decolorization of simulated textile effluent by recombinant laccase[J]. Applied Microbiology and Biotechnology, 2017, 101(2):685-696.
