產(chǎn)品詳情介紹
本產(chǎn)品含有反轉(zhuǎn)錄反應(yīng)所需的全部試劑(TransScript? RT, RNase Inhibitor, Anchored Oligo(dT)18 Primer, Random Primer(N9), dNTPs, Buffer)，濃度為5×。反應(yīng)時(shí)，只需加入gDNA Remover、模板RNA和水即可高效地合成第一鏈cDNA，同時(shí)去除RNA模板中殘留的基因組DNA。另配有5×TransScript? All-in-One No-RT Control SuperMix for qPCR，用于配制無反轉(zhuǎn)錄酶的對照，判斷qPCR模板是否來自cDNA。該產(chǎn)品操作簡便，降低操作過程中的污染機(jī)率。cDNA只適用于qPCR，不適用于常規(guī)PCR。
使用前請將各組分點(diǎn)甩離心
1 Sun X, Zhang T, Tong B, et al. POGZ suppresses 2C transcriptional program and retrotransposable elements[J]. Cell Reports, 2023.(IF 8.80)
2 Yuan F, Cai J, Wu J, et al. Z-DNA binding protein 1 promotes heatstroke-induced cell death[J]. Science, 2022.(IF 47.72)
3 Yuan F, Cai J, Wu J, et al. Z-DNA binding protein 1 promotes heatstroke-induced cell death[J]. Science, 2022.(IF 63.71)
4 Yu Z, Deng P, Chen Y, et al. Inhibition of the PLK1‐coupled cell cycle machinery overcomes resistance to oxaliplatin in colorectal cancer[J]. Advanced Science, 2021.(IF 16.80)
5 Sun X, Peng X, Cao Y, et al. ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling[J]. Nature communications, 2020.(IF 12.12)
6 Wu H, Qu X, Dong Z, et al. WUSCHEL triggers innate antiviral immunity in plant stem cells[J]. Science, 2020.(IF 41.84)
7 Ji S, Luo Y, Cai Q, et al. LC domain-mediated coalescence is essential for Otu enzymatic activity to extend Drosophila lifespan[J]. Molecular cell, 2019.(IF 14.20)
